Effect of preconditioning and postoperative hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats
Acta cir. bras
; 35(5): e202000503, 2020. tab
Article
em En
| LILACS
| ID: biblio-1130645
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose To investigate the effect of hyperbaric oxygen therapy on colonic anastomosis healing with and without ischemia in rats. Methods Forty female rats underwent segmental resection of 1 cm of the left colon followed by end-to-end anastomosis. They were randomly assigned to four groups (n=10 each), a sham group; two groups were submitted to Hyperbaric Oxygen therapy (HBOT) with and without induced ischemia and the induced ischemia group without HBOT. The HBOT protocol evaluated was 100% O2 at 2.4 Atmosphere absolute pressure (ATA) for 60 minutes, two sessions before as a preconditioning protocol and three sessions after the operation. Clinical course and mortality were monitored during all experiment and on the day of euthanasia on the fourth day after laparotomy. Macroscopic appearance of the abdominal cavity were assessed and samples for breaking strength of the anastomosis and histopathological parameters were collected. Results There was no statistically significant difference in mortality or anastomosis leak between the four experimental groups. Anastomosis breaking strength was similar across groups. Conclusion The HBOT protocol tested herein at 2.4 ATA did not affect histopathological and biomechanical parameters of colonic anastomotic healing, neither the clinical outcomes death and anastomosis leak on the fourth day after laparotomy.
Palavras-chave
Texto completo:
1
Base de dados:
LILACS
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
/
Oxigenoterapia_hiperbrica
Assunto principal:
Cicatrização
/
Colo
/
Precondicionamento Isquêmico
/
Oxigenoterapia Hiperbárica
/
Isquemia
Tipo de estudo:
Guideline
Idioma:
En
Revista:
Acta cir. bras
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil