The cDNA sequences of equine antioxidative enzyme genes Cu/Zn-SOD and Mn-SOD, and these expressions in equine tissues.
J Vet Med Sci
; 61(3): 291-4, 1999 Mar.
Article
em En
| MEDLINE
| ID: mdl-10331206
The entire cDNA sequences were determined by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) techniques for equine copper/zinc superoxide dismutase (Cu/Zn-SOD) and manganese superoxide dismutase (Mn-SOD) through the use of total RNA extracted from the testis of an adult Thoroughbred. The results revealed a protein coding region for equine Cu/Zn-SOD with bases totaling 465 bp, accompanied by an estimated 154 residues of amino acids. As for equine Mn-SOD, its coding region contained a total of 669 bp and an estimated 222 residues of amino acids. Further, the expression of Cu/Zn-SOD and Mn-SOD genes were confirmed in the equine tissues by RT-PCR and in situ hybridization.
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Base de dados:
MEDLINE
Assunto principal:
Superóxido Dismutase
/
Regulação Enzimológica da Expressão Gênica
/
Cavalos
Idioma:
En
Revista:
J Vet Med Sci
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Japão