Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells.
J Ethnopharmacol
; 66(2): 227-33, 1999 Aug.
Article
em En
| MEDLINE
| ID: mdl-10433483
ABSTRACT
The enzyme cyclooxygenase-2 (COX-2) is abundantly expressed in colon cancer cells and plays a key role in colon tumorigenesis. Compounds inhibiting COX-2 transcriptional activity have therefore potentially a chemopreventive property against colon tumor formation. An assay method for estimating COX-2 transcriptional activity in human colon cancer cells was established using a beta-galactosidase reporter gene system, and examination was made of various medicinal herbs and their ingredients for an inhibitory effect on COX-2 transcriptional activity. We found that berberine, an isoquinoline alkaloid present in plants of the genera Berberis and Coptis, effectively inhibits COX-2 transcriptional activity in colon cancer cells in a dose- and time-dependent manner at concentrations higher than 0.3 microM. The present findings may further explain the mechanism of anti-inflammatory and anti-tumor promoting effects of berberine.
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Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Berberina
/
Prostaglandina-Endoperóxido Sintases
/
Neoplasias do Colo
/
Isoenzimas
Idioma:
En
Revista:
J Ethnopharmacol
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Japão