Printing proteins as microarrays for high-throughput function determination.
Science
; 289(5485): 1760-3, 2000 Sep 08.
Article
em En
| MEDLINE
| ID: mdl-10976071
ABSTRACT
Systematic efforts are currently under way to construct defined sets of cloned genes for high-throughput expression and purification of recombinant proteins. To facilitate subsequent studies of protein function, we have developed miniaturized assays that accommodate extremely low sample volumes and enable the rapid, simultaneous processing of thousands of proteins. A high-precision robot designed to manufacture complementary DNA microarrays was used to spot proteins onto chemically derivatized glass slides at extremely high spatial densities. The proteins attached covalently to the slide surface yet retained their ability to interact specifically with other proteins, or with small molecules, in solution. Three applications for protein microarrays were demonstrated screening for protein-protein interactions, identifying the substrates of protein kinases, and identifying the protein targets of small molecules.
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Base de dados:
MEDLINE
Assunto principal:
Ligação Proteica
/
Proteínas Quinases
/
Bioquímica
/
Proteínas
/
Técnicas de Sonda Molecular
Idioma:
En
Revista:
Science
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos