Factorial designs combined with the steepest ascent method to optimize serum-free media for CHO cells.
Enzyme Microb Technol
; 28(4-5): 314-321, 2001 Mar 08.
Article
em En
| MEDLINE
| ID: mdl-11240185
ABSTRACT
A serum free medium for recombinant CHO NTHU 108 cell growth and fusion protein (CD20 linked to a human IgG-Fc gamma4 fragment) synthesis were systematically developed using factorial designs combined with the steepest ascent method. Experimental results indicate that the optimal composition of serum replacement for specific fusion protein production was 1% SITE (selenium, insulin, transferrin, ethanolamine), 0.3 g/L yeast extract, and 0.09% linoleic acid-BSA. Cell growth and fusion protein production of the adapted CHO NTHU 108 cultured in Iscove's modified Dulbecco's medium supplemented with these serum substitutes were comparable to those in the Ex-Cell 301 commercial serum-free medium. These serum substitutes can also promote CHO cell growth and fusion protein production in nine kinds of commercial media. The low protein content of the developed medium facilitates downstream processing and product purification.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Enzyme Microb Technol
Ano de publicação:
2001
Tipo de documento:
Article