[Experimental study on inhibitory effect of Chinese herbal compound on hepatocyte cytochrome P450 II E1 expression in rat model of nonalcoholic fatty liver].

ABSTRACT

OBJECTIVE: To study the effect of Chinese herbal compound (CHC) on hepatocyte cytochrome P450 II E1 expression in rat model of nonalcoholic fatty liver (NFL). METHODS: CHC was given to the models of NFL formed by high fat diet, and changes on pathology of liver and hepatocyte cytochrome P450 II E1 expression, as well as the contents of malonyldialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), vitamin E (VitE) and triglyceride (TG) in liver were observed and compared with those in the control group. RESULTS: The fatty degeneration of liver recovered to normal basically in the CHC group. Immunohistochemical examination showed that CHC could inhibit the hepatocyte cytochrome P450 II E1 expression markedly, and restore the liver contents of MDA, SOD, GSH, VitE and TG to near normal range. CONCLUSION: CHC could inhibit the hepatocyte cytochrome P450 II E1 expression markedly in rats with fatty liver, therefore, has the effect of preventing fatty liver.