Considerations for using fluorescence polarization in the screening of G protein-coupled receptors.
J Biomol Screen
; 7(2): 111-7, 2002 Apr.
Article
em En
| MEDLINE
| ID: mdl-12006109
ABSTRACT
Fluorescence polarization must be classified as a unique detection method relative to other intensity-based methods because both bound and unbound tracer is measured. Other fluorescence techniques require either physical removal of unbound tracer or a means for distinguishing only bound tracer. The presence of unbound tracer in the detection volume has profound consequences on how assay performance is gauged. The intent here is to provide tools for accurate assay performance assessment and to discuss some of the practical considerations necessary for understanding the advantages and limitations of the technology. An emphasis is placed on applications using G protein-coupled receptors.
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Base de dados:
MEDLINE
Assunto principal:
Receptores de Superfície Celular
/
Proteínas de Ligação ao GTP
/
Avaliação Pré-Clínica de Medicamentos
/
Polarização de Fluorescência
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
/
Screening_studies
Idioma:
En
Revista:
J Biomol Screen
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Canadá