Determination of matrine and oxymatrine in Sophora subprostata by CE.
J Pharm Biomed Anal
; 28(5): 1005-10, 2002 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-12039644
ABSTRACT
Shan-dou-gen is the dried roots of Sophora subprostata (Leguminosae) and a commonly used Chinese herbal drug in Taiwan. It possesses antipyretic, anti-inflammatory, analgesic effects and is used to treat sore throat and acute pharyngolaryngeal infections. To evaluate the quality of S. subprostata, a simple, rapid and accurate high-performance capillary electrophoresis (HPCE) method was developed for the assay of two alkaloids matrine and oxymatrine. The electrolyte was a buffer solution containing 75% 130 mM phosphate buffer (NaH2PO4/H3PO4, pH 3.5) and 25% acetonitrile. Applied voltage was 10 kV and temperature was 30 degrees C. 2-(4-Hydroxyphenyl)ethylammonium chloride was used as an internal standard and detector set at 200 nm. The contents of matrine and oxymatrine of S. subprostata in several different samples of crude drugs and commercial concentrated preparation have also been determined.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Alcaloides
/
Fabaceae
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2002
Tipo de documento:
Article