Toxicity of hexavalent chromium and its reduction by bacteria isolated from soil contaminated with tannery waste.
Curr Microbiol
; 47(1): 51-4, 2003 Jul.
Article
em En
| MEDLINE
| ID: mdl-12783193
ABSTRACT
An Arthrobacter sp. and a Bacillus sp., isolated from a long-term tannery waste contaminated soil, were examined for their tolerance to hexavalent chromium [Cr(VI)] and their ability to reduce Cr(VI) to Cr(III), a detoxification process in cell suspensions and cell extracts. Both bacteria tolerated Cr(VI) at 100 mg/ml on a minimal salts agar medium supplemented with 0.5% glucose, but only Arthrobacter could grow in liquid medium at this concentration. Arthrobacter sp. could reduce Cr(VI) up to 50 microg/ml, while Bacillus sp. was not able to reduce Cr(VI) beyond 20 microg/ml. Arthrobacter sp. was distinctly superior to the Bacillus sp. in terms of their Cr(VI)-reducing ability and resistance to Cr(VI). Assays with permeabilized (treated with toluene or Triton X 100) cells and crude extracts demonstrated that the Cr(VI) reduction was mainly associated with the soluble protein fraction of the cell. Arthrobacter sp. has a great potential for bioremediation of Cr(VI)-containing waste.
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Base de dados:
MEDLINE
Assunto principal:
Arthrobacter
/
Microbiologia do Solo
/
Bacillus
/
Cromo
Idioma:
En
Revista:
Curr Microbiol
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Austrália