Beijerinckia indica var. penicillanicum penicillin V acylase: enhanced enzyme production by catabolite repression-resistant mutant and effect of solvents on enzyme activity.
J Ind Microbiol
; 7(3): 209-14, 1991 Apr.
Article
em En
| MEDLINE
| ID: mdl-1367509
ABSTRACT
Beijerinckia indica var. penicillanicum mutant UREMS-5, producing 168% more penicillin V acylase, was obtained by successive treatment with UV, gamma-irradiation and ethylmethane sulfonate. Penicillin V acylase production by the mutant strain was resistant to catabolite repression by glucose. Incorporation of glucose, sodium glutamate and vegetable oils in the medium enhanced enzyme production. The maximum specific production of penicillin V acylase was 244 IU/g dry weight of cells. Effect of solvents on hydrolysis of penicillin V by soluble penicillin V acylase and whole cells was studied. Methylene chloride, chloroform and carbon tetrachloride significantly stimulated the rate of penicillin V hydrolysis by whole cells.
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Base de dados:
MEDLINE
Assunto principal:
Penicilina Amidase
/
Pseudomonadaceae
/
Solventes
Idioma:
En
Revista:
J Ind Microbiol
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Índia