A protein tyrosine kinase in the interferon alpha/beta signaling pathway.
Cell
; 70(2): 313-22, 1992 Jul 24.
Article
em En
| MEDLINE
| ID: mdl-1386289
ABSTRACT
The mutant human cell line 11.1 is unresponsive to interferon alpha. Here we describe the genetic complementation of this mutant and the identification and cloning of the wild-type gene that corrects the defect. Using transfection with genomic DNA in conjunction with a powerful back-selection, we isolated a cosmid that reverts the mutant phenotype of 11.1 cells. The cosmid encodes a single message whose level is greatly reduced in mutant cells. Complementary DNAs were cloned and found to be virtually identical to tyk2, a human mRNA encoding a non-receptor protein tyrosine kinase of previously unknown function. This finding shows that tyk2 links the interferon alpha/beta receptor to the cytoplasmic transcription factor that mediates activation of interferon-responsive genes.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Tirosina Quinases
/
Transdução de Sinais
/
Regulação da Expressão Gênica
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Cell
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França