[Studies on establishing rRNA gene map of Angelica sinensis and Rheum palmatum from Gansu by DNA sequencing].

ABSTRACT

OBJECTIVE: To amplify and sequence the internal transcripted spacer of rRNA gene of the seeds of Angelica sinensis and Rheum palmatum for establishing a new method to identify the Chinese herb seeds in molecular level. METHODS: rDNA in the seeds was extracted. Specific synthesized primers were applied to amplify the internal transcripted spacer of rRNA gene by nested PCR. And the PCR products were sequenced. RESULTS: PCR results were confirmed by agarose electrophoresis, the above-mentioned methods could achieve the PCR products of interval region of rRNA gene in the seeds, and the sequences of the internal transcripted spacer of rRNA gene in the seeds were acquired by sequencing. There existed marked difference. CONCLUSION: The base sequences of the internal transcripted spacer of rRNA gene in different plant Chinese seeds can be applied to be identifying marker in molecular level.