Metabolic engineering of medicinal plants: transgenic Atropa belladonna with an improved alkaloid composition.
Proc Natl Acad Sci U S A
; 89(24): 11799-803, 1992 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-1465402
ABSTRACT
The tropane alkaloid scopolamine is a medicinally important anticholinergic drug present in several solanaceous plants. Hyoscyamine 6 beta-hydroxylase (EC 1.14.11.11) catalyzes the oxidative reactions in the biosynthetic pathway leading from hyoscyamine to scopolamine. We introduced the hydroxylase gene from Hyoscyamus niger under the control of the cauliflower mosaic virus 35S promoter into hyoscyamine-rich Atropa belladonna by the use of an Agrobacterium-mediated transformation system. A transgenic plant that constitutively and strongly expressed the transgene was selected, first by screening for kanamycin resistance and then by immunoscreening leaf samples with an antibody specific for the hydroxylase. In the primary transformant and its selfed progeny that inherited the transgene, the alkaloid contents of the leaf and stem were almost exclusively scopolamine. Such metabolically engineered plants should prove useful as breeding materials for obtaining improved medicinal components.
Texto completo:
1
Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
Assunto principal:
Plantas Medicinais
/
Plantas Tóxicas
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Atropina
/
Escopolamina
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Atropa belladonna
/
Plantas Geneticamente Modificadas
/
Oxigenases de Função Mista
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Japão