Screening for the breast cancer gene (BRCA1) using a biochip system and molecular beacon probes immobilized on solid surfaces.
J Biomed Opt
; 9(3): 439-43, 2004.
Article
em En
| MEDLINE
| ID: mdl-15189080
ABSTRACT
We describe the use of a biochip based on complementary metal oxide semiconductor (CMOS) technology for detection of specific genetic sequences using molecular beacons (MB) immobilized on solid surfaces as probes. The applicability of this miniature detection system for screening for the BRCA1 gene is evaluated using MB probes, designed especially for the BRCA1 gene. MB probes are immobilized on a zeta-probe membrane by biotin-streptavidin immobilization. Two immobilization strategies are investigated to obtain optimal assay sensitivity. The MB is immobilized by manual spotting on zeta-probe membrane surfaces with the use of a custom-made stamping system. The detection of the BRCA1 gene using an MB probe is successfully demonstrated and expands the use of the CMOS biochip for medical applications.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Sondas de Oligonucleotídeos
/
Biomarcadores Tumorais
/
Testes Genéticos
/
Proteína BRCA1
/
Análise de Sequência com Séries de Oligonucleotídeos
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
/
Screening_studies
Idioma:
En
Revista:
J Biomed Opt
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos