[Effect of acupuncture serum on intracellular Ca2+ concentration in cultured neurons].
Zhong Xi Yi Jie He Xue Bao
; 2(6): 453-5, 2004 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-15539028
ABSTRACT
OBJECTIVE:
To study the humoral factor of acupuncture in improving functional activities of the organism.METHODS:
Neurons of the cerebral cortex from the new born rats were taken to be cultured in a strictly controlled medium. Seven to ten days later, fluorescein-molecular probe Fluo-3AM was used for staining intracellular Ca(2+). Ca(2+) concentration and its changes after application of acupuncture serum were measured by using a laser confocal microscope. Normal serum and acupuncture serum were derived from the normal control rats and the rats undergoing electro-acupuncture of "Baihui (GV 20)", "Zusanli (ST 36)", "Quchi (LI 11)" and "Sanyinjiao (SP 6)" for 2 weeks.RESULTS:
After application of normal serum, the intracellular Ca(2+) concentration increased strikingly immediately at the beginning, and gradually got stable afterwards. When acupuncture serum was added to the culture fluid, Ca(2+) concentration lowered significantly.CONCLUSION:
These results suggest that bioactive substances in serum collected from rats undergoing acupuncture can affect the intracellular Ca(2+) concentration of the cerebral neurons, and the humoral factor may participate in the effect of acupuncture in regulating functional activities of the organism.
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Base de dados:
MEDLINE
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Métodos Terapêuticos e Terapias MTCI:
Terapias_energeticas
Assunto principal:
Eletroacupuntura
/
Cálcio
/
Neurônios
Idioma:
Zh
Revista:
Zhong Xi Yi Jie He Xue Bao
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China