Capillary zone electrophoresis as a tool for the quality control of goldenseal extracts.
Electrophoresis
; 26(12): 2430-6, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-15912542
ABSTRACT
The root extracts of goldenseal (Hydrastis canadensis L.) are popular phytomedicines for the treatment of gastrointestinal disorders and upper respiratory tract infections. Here we describe a simple and fast capillary zone electrophoresis (CZE) method with ultraviolet detection at 225 nm for the quantification of the major goldenseal constituents, berberine and hydrastine, in herbal remedies containing goldenseal root extracts. Tritoqualine, an antihistaminic drug with a hydrastine-like phthalidisoquinoline structure, was applied as an internal standard. The running buffer was a 15 mixture of 500 mM ammonium acetate (adjusted to pH 3.4 with acetic acid) and methanol. Our newly developed CZE method was validated regarding limit of detection (LOD), limit of quantification, linearity, accuracy and precision. For both berberine and hydrastine, the LOD was 1.0 microg/mL and the linearity was obtained between 2.5 and 500 microg/mL. Using our newly developed method, both the alkaloids could be analysed in herbal remedies containing goldenseal root extracts within 8 min.
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Base de dados:
MEDLINE
Assunto principal:
Berberina
/
Extratos Vegetais
/
Hydrastis
/
Alcaloides
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Alemanha