The Arabidopsis TFIID factor AtTAF6 controls pollen tube growth.
Dev Biol
; 285(1): 91-100, 2005 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-16039640
ABSTRACT
Initiation of transcription mediated by RNA polymerase II requires a number of transcription factors among which TFIID is the major core promoter recognition factor. TFIID is composed of highly conserved factors which include the TATA-binding protein (TBP) and about 14 TBP-associated factors (TAFs). Recently, the complete Arabidopsis TAF family has been identified. To obtain functional information about Arabidopsis TAFs, we analyzed a T-DNA insertion mutant for AtTAF6. Segregation analysis showed that plants homozygous for the mutant allele were never found, indicating that inhibition of the AtTAF6 function is lethal. Genetic experiments also revealed that the male gametophyte was affected by the attaf6 mutation since significant reduced transmission of the mutant allele through the male gametophyte was observed. Detailed histological and morphological analysis showed that the T-DNA insertion in AtTAF6 specifically affects pollen tube growth, indicating that the transcriptional regulation of only a specific subset of genes is controlled by this basal transcription factor.
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Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
/
Proteínas de Arabidopsis
/
Fator de Transcrição TFIID
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Dev Biol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Itália