Effects of hydrothermal treatment with CaCl(2) solution on surface property and cell response of titanium implants.

ABSTRACT

In order to obtain early and good osteointegration after implantation of a titanium implant in the human body, the surface modified treatments using NaOH or H(2)O(2) etc. were reported. In this study, titanium was hydrothermally treated with CaCl(2) solutions at 200 degrees C for 24hr (CaCl(2)-HT). Scanning electron microscope (SEM) observation clearly showed apatite deposition on the surface of CaCl(2) HT treated titanium faster than other chemical treated titanium immersion in simulated body fluid. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that Ti--O--Ca bonding was formed on titanium surface by hydrothermal treatment with CaCl(2) solution. And it was revealed that thickness of TiO(2), which was known to play important roles for the formation of bone-like apatite, became approximately three times thicker than as-polished titanium. The amount of initial attached MC3T3-E1 cells on as-polished and NaOH, H(2)O(2) and this CaCl(2) HT treated titanium were almost the same values. After 5 days incubation, the growth rate of MC3T3-E1 cells on CaCl(2)-HT treated titanium was significantly higher than that on other chemical treated titanium. The hydrothermal treatment with 10-20 mmol/L CaCl(2) solution at 200 degrees C was an effective method for the fabrication of titanium implant with good bioactivity and osteoconductivity.