Omega-3 fatty acids and decidual cell prostaglandin production in response to the inflammatory cytokine IL-1beta.
Am J Obstet Gynecol
; 195(6): 1693-9, 2006 Dec.
Article
em En
| MEDLINE
| ID: mdl-16792994
ABSTRACT
OBJECTIVE:
The objective of this study was to determine the effect of omega-3 fatty acids (eicosapentaenoic acid [EPA]; docosahexaenoic acid [DHA]) on prostaglandin production and prostanoid enzyme expression in cultured decidual cells exposed to interleukin-1beta (IL-1beta), a cytokine that plays a major role in inflammation. STUDYDESIGN:
Decidua was obtained from human placentas of nonlaboring patients at term cesarean delivery (N = 6) and cultured by using standard cell culture techniques. Cells were preincubated in defined media with various concentrations of vehicle, DHA, or EPA for 1 hour. IL-1beta (10 ng/mL) was then added to the media, and experiments were terminated 12 hours after exposure to IL-1beta. Prostaglandin E2 (PGE2) and PGF2alpha concentrations in conditioned media were measured by enzyme-linked immunosorbent assay; cyclooxygenase-1 (COX-1), COX-2, microsomal prostaglandin E synthase (mPGES)-1, mPGES-2, and 15-hydroxy prostaglandin dehydrogenase (PGDH) expression were quantified by real-time polymerase chain reaction and immunoblotting. Groups were compared with the use of Student t test, with significance defined as P < .05.RESULTS:
Preincubation with DHA decreased prostaglandin production by up to 80% when compared with controls. DHA decreased both mPGES-1 and -2 messenger RNA expression by approximately 50% (P = .02). Preincubation in DHA or EPA had no effect on COX-1, COX-2, and PGDH messenger RNA or protein expression.CONCLUSION:
Under conditions simulating inflammation, supplementation with omega-3 fatty acids decreases PGE2 and PGF2alpha production in cultured decidual cells. The reduction in prostaglandin production was associated with a decreased expression of mPGES-1 and -2. These findings suggest a mechanism by which omega-3 fatty acid supplementation decreases the incidence of preterm birth in high-risk patients.
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Base de dados:
MEDLINE
Assunto principal:
Antagonistas de Prostaglandina
/
Prostaglandinas
/
Ácido Eicosapentaenoico
/
Ácidos Docosa-Hexaenoicos
/
Mediadores da Inflamação
/
Decídua
/
Interleucina-1beta
Idioma:
En
Revista:
Am J Obstet Gynecol
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos