Role of bovine albumin in a serum-free suspension cell culture medium.
Proc Soc Exp Biol Med
; 149(2): 439-42, 1975 Jun.
Article
em En
| MEDLINE
| ID: mdl-168586
ABSTRACT
A serum-free culture medium, supplemented with 1% bovine serum albumin, supported the growth of both primary and continuous suspension-type cultures of various mammalian tumor cells. The role of albumin added to the medium was also studied. Defatted albumin failed to support cell growth, unless reconstituted with its lipid extract. Similarly, defatted albumin when combined with oleic and linoleic acids, also supported cell growth. Therefore, albumin-bound fatty acids play an important growth-promoting role in serum-free medium.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Soroalbumina Bovina
/
Células Cultivadas
/
Meios de Cultura
Idioma:
En
Revista:
Proc Soc Exp Biol Med
Ano de publicação:
1975
Tipo de documento:
Article