Apoptosis and nuclear proliferation in rat small bowel submitted to hypothermic hyperbaric oxygenation for preservation.
Transplant Proc
; 38(6): 1876-8, 2006.
Article
em En
| MEDLINE
| ID: mdl-16908311
ABSTRACT
UNLABELLED This study was conducted to assess apoptosis and nuclear proliferation in rat small bowel submitted to hypothermic hyperbaric oxygenation for preservation. METHODS:
Twenty two-month-old, male Wistar rats, weighing 250 g were divided into two groups group I (n = 10), in which the small bowel was preserved for 12 hours, and group II (n = 10) in which the small bowel was preserved for 24 hours. After vascular and intraluminal perfusion, 3-cm segments were maintained in Ringer's solution at 2 degrees to 4 degrees C under normobaric conditions (groups Ia and IIa) or conditioned in a small hyperbaric metal chamber with 100% oxygen at 5.5 absolute atmospheres (groups Ib and IIb). After 12 or 24 hours, apoptotic and mitotic indices were evaluated by immunohistochemical methods.RESULTS:
The apoptotic index was significantly higher in small bowel segments in groups Ia and IIa compared with groups Ib and IIb. The mitotic index was significantly higher among group IIb.CONCLUSION:
Hypothermic hyperbaric oxygenation reduced intestinal epithelial apoptosis and increased nuclear proliferation during rat small bowel preservation.
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Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
/
Oxigenoterapia_hiperbrica
Assunto principal:
Preservação de Órgãos
/
Núcleo Celular
/
Apoptose
/
Intestino Delgado
Idioma:
En
Revista:
Transplant Proc
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Brasil