Flavonoids with DNA strand-scission activity from Rhus javanica var. roxburghiana.
Fitoterapia
; 79(1): 32-6, 2008 Jan.
Article
em En
| MEDLINE
| ID: mdl-17855017
ABSTRACT
The flavonoids isolated from the stems of Rhus javanica var. roxburghiana, taxifolin (1), fisetin (2), fustin (3), 3,7,4'-trihydroxyflavanone (4) and 3,7,4'-trihydroxyflavone (5) caused breakage of supercoiled plasmid pBR322 DNA in the presence of Cu(II). Cu(I) was shown to be an essential intermediate by using the Cu(I)-specific sequestering reagent neocuproine. The Cu(II)-mediated DNA scissions induced by 1, 2, 3 and 5 were inhibited by the addition of catalase and exhibited DNA strand break by the addition of KI and superoxide dimutase (SOD), while in the Cu(II)-mediated DNA scissions induced by 4 was inhibited by the addition of KI, SOD, and catalase. It is concluded that 1, 2, 3, and 5 can induce H2O2 and superoxide anion, while 4 can induce OH* and H2O2 and subsequent oxidative damage of DNA in the presence of Cu(II).
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Base de dados:
MEDLINE
Assunto principal:
Flavonoides
/
Dano ao DNA
/
DNA Super-Helicoidal
/
Extratos Vegetais
/
Rhus
/
Mutagênicos
Idioma:
En
Revista:
Fitoterapia
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Taiwan