Separation and quantification of PGE3 following derivatization with panacyl bromide by high pressure liquid chromatography with fluorometric detection.
Prostaglandins
; 42(4): 355-68, 1991 Oct.
Article
em En
| MEDLINE
| ID: mdl-1796150
ABSTRACT
Separation of prostaglandin E3 (PGE3) from prostaglandin E2 (PGE2) and prostaglandin E1 (PGE1) was achieved following derivatization with p-(9-anthroyloxy)phenacyl bromide (panacyl bromide). The eicosanoid esters were analysed by reverse phase high pressure liquid chromatography with fluorometric detection (excitation 360nm and emission 470nm). Human, rat and mouse adherent cells were incubated overnight and the culture medium extracted, derivatized and analysed for PG production. PGE2 was detected from biological samples of each species tested. PGE2 synthesis was reduced when cells were incubated overnight with 5 microM eicosapentaenoic acid. PGE3 was not detectable under these experimental conditions. Studies were also undertaken using adherent cells from mice, rats and human subjects given dietary fish oil supplements rich in EPA. PGE3 production by these cells was not detected although the dietary regimens yielded substantial incorporation of EPA into cell membranes and leukocyte LTB5 production was demonstrable.
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Base de dados:
MEDLINE
Assunto principal:
Alprostadil
/
Dinoprostona
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Prostaglandins
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Austrália