Anti-histone acetyltransferase activity from allspice extracts inhibits androgen receptor-dependent prostate cancer cell growth.
Biosci Biotechnol Biochem
; 71(11): 2712-9, 2007 Nov.
Article
em En
| MEDLINE
| ID: mdl-17986787
ABSTRACT
Histone acetylation depends on the activity of two enzyme families, histone acetyltransferase (HAT) and deacetylase (HDAC). In this study, we screened various plant extracts to find potent HAT inhibitors. Hot water extracts of allspice inhibited HAT activity, especially p300 and CBP (40% at 100 microg/ml). The mRNA levels of two androgen receptor (AR) regulated genes, PSA and TSC22, decreased with allspice treatment (100 microg/ml). Importantly, in IP western analysis, AR acetylation was dramatically decreased by allspice treatment.Furthermore, chromatin immunoprecipitation indicated that the acetylation of histone H3 in the PSA and B2M promoter regions was also repressed. Finally, allspice treatment reduced the growth of human prostate cancer cells, LNCaP (50% growth inhibition at 200 microg/ml). Taken together, our data indicate that the potent HAT inhibitory activity of allspice reduced AR and histone acetylation and led to decreased transcription of AR target genes, resulting in inhibition of prostate cancer cell growth.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
/
Pimenta
/
Inibidores Enzimáticos
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Histona Acetiltransferases
/
Antagonistas de Receptores de Andrógenos
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Antineoplásicos
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Ano de publicação:
2007
Tipo de documento:
Article