Induction of apoptosis in HepG2 cells by solanine and Bcl-2 protein.
J Ethnopharmacol
; 115(2): 194-202, 2008 Jan 17.
Article
em En
| MEDLINE
| ID: mdl-18022776
ABSTRACT
The nightshade (Solanum nigrum Linn.) has been widely used in Chinese traditional medicine as a remedy for the treatment of digestive system cancer. The anti-tumor activity of solanine, a steroid alkaloid isolated from the nightshade has been demonstrated. To observe the effect of anti-tumor and mechanism of solanine. The MTT assay was used to evaluate the IC(50) on the three digestive system tumor cell lines. The effect on the morphology was observed with a laser confocal microscopy; the rate of apoptosis and the cell cycle were measured using flow cytometry (FCM); the expression of Bcl-2 protein was measured by Western blot. The results show that the IC(50) for HepG(2), SGC-7901, and LS-174 were 14.47, >50, and >50 microg/ml, respectively; the morphology of cells in the negative control was normal; for the treated groups, typical signs for apoptosis were found. The rate of apoptosis in HepG(2) cells induced by solanine was found to be 6.0, 14.4, 17.3, 18.9, and 32.2%, respectively. Observation of the cell cycle showed that cells in the G(2)/M phases disappeared while the number of cells in the S phase increased significantly for treated groups. Western blot showed that solanine decreased the expression of Bcl-2 protein. Therefore, the target of solanine in inducing apoptosis in HepG(2) cells seems to be mediated by the inhibition in the expression of Bcl-2 protein.
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Base de dados:
MEDLINE
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Métodos Terapêuticos e Terapias MTCI:
Plantas_medicinales
Assunto principal:
Solanina
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Neoplasias do Sistema Digestório
/
Antineoplásicos Fitogênicos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Ethnopharmacol
Ano de publicação:
2008
Tipo de documento:
Article