In vitro anti-inflammatory effects of different solution fractions of ethanol extract from Melilotus suaveolens Ledeb.
Chin Med J (Engl)
; 120(22): 1992-8, 2007 Nov 20.
Article
em En
| MEDLINE
| ID: mdl-18067784
ABSTRACT
BACKGROUND:
Melilotus suaveolens Ledeb (M. suaveolens Ledeb) has long been used as a folk medicine in inflammation-related therapy. This study was undertaken to determine the anti-inflammatory effect of the plant.METHODS:
Petroleum ether fraction, ethyl acetate fraction, n-butanol fraction, aqueous fraction were obtained from ethanol extract of M. suaveolens Ledeb and evaluated by high performance liquid chromatography (HPLC). While dexamethasone (DM) was used as a positive control, the effects of different solution fractions of ethanol extract on tumor necrosis factor alpha (TNF-alpha) mRNA, cyclooxygenase 2 (COX-2) mRNA, COX-2 and nuclear factor kappaB (NF-kappaB) of LPS-stimulated RAW 264.7 cells were studied by real-time PCR, Western blot analysis and immunocytochemical assay, respectively.RESULTS:
Coumarin was one of the main ingredients in different solution fractions of ethanol extract except the aqueous fraction with no inflammatory effect. The petroleum ether fraction, ethyl acetate fraction and n-butanol fraction of ethanol extract could inhibit the production of TNF-alpha mRNA, COX-2 mRNA and NF-kappaB to some extent.CONCLUSIONS:
Different solution fractions of ethanol extract from M. suaveolens Ledeb had similar anti-inflammatory effect as did dexamethasone except the aqueous fraction. Coumarin was likely to be essential to the anti-inflammatory effect, and other ingredients might attribute to their different anti-inflammatory effects from the HPLC fingerprint.
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Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Anti-Inflamatórios não Esteroides
/
Melilotus
Idioma:
En
Revista:
Chin Med J (Engl)
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China