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In situ comparative studies on subnucleolar distribution and configuration of plant rDNA.
Long, Hong; He, Jie; Sun, Haijing; Hao, Shui; Jiao, Mingda.
Afiliação
  • Long H; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Micron ; 39(4): 405-10, 2008 Jun.
Article em En | MEDLINE | ID: mdl-18262427
The distribution and configurations of nucleolar DNA in Pisum sativum L., Allium sativum L., Triticum aestivum L. were analyzed by specific cytochemical staining using NAMA-Ur. It has been observed that in the nucleoli of different plant species, the DNA occupied different positions in different areas, which may imply a different status and strategy of rDNA transcription. Our results showed irregular clumps of rDNA surrounding FCs in semi-circular formations in P. sativum and T. aestivum, indicating a regular pattern of rDNA distribution and supporting the helix model of rDNA configuration. The rDNA was condensed in some regions and uncondensed in others. Nucleolus-associated chromatin extended from outside the nucleolus to the periphery of the FCs via nucleolar channels, which suggests a possible origin for nucleolar DNA.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA Ribossômico / Nucléolo Celular / DNA de Plantas Idioma: En Revista: Micron Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA Ribossômico / Nucléolo Celular / DNA de Plantas Idioma: En Revista: Micron Ano de publicação: 2008 Tipo de documento: Article