Characterization of a dammarenediol synthase in Centella asiatica (L.) Urban.
Plant Physiol Biochem
; 47(11-12): 998-1002, 2009.
Article
em En
| MEDLINE
| ID: mdl-19796960
ABSTRACT
To elucidate the exact function of CabAS in Centella asiatica, which was previously reported as a putative beta-amyrin synthase [Plant Cell Rep, 24304-311, 2005], this gene was functionally expressed in the lanosterol synthase-deficient yeast mutant (erg7). After inducing the CabAS gene with galactose, a peak consistent with the dammarenediol standard was detected in LC/APCIMS analyses and the accumulated product was confirmed as dammarenediol. CabAS should therefore be renamed to C. asiatica dammarenediol synthase (CaDDS). The confirmation of this gene function may allow us to better understand the generation of numerous triterpene carbon skeletons.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Saponinas
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Expressão Gênica
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Genes de Plantas
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Alquil e Aril Transferases
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Centella
Idioma:
En
Revista:
Plant Physiol Biochem
Ano de publicação:
2009
Tipo de documento:
Article