[Extraction, isolation and purification for ginkgolide B].

OBJECTIVE: To establish a simple extraction, isolation and purification method for ginkgolide B from ginkgo leaf. METHOD: The optimum conditions of extraction, isolation and purification were studied by taking the transfer rate of ginkgolide B as index. RESULT: Ginkgo leaf was extracted with 70% ethanol for three times, the extracts were concentrated to remove ethanol and diluted by water till the crude drug density reached 0.1 g x mL(-1). The dilution was adsorbed with HPD-450 macroporous resin. The impurities were eluted with 20% ethanol and ginkgolide B was eluted with 80% ethanol. Then the 80% ethanol eluant was concentrated and crystallized. Finally the crude crystals were recrystallized with isopropanol. The purity of the ginkgolide B recrystallization was 95%. CONCLUSION: The process was stable and easy to operate, which was suited to industrialized production.