[Effects of moxibustion on the expression of cell cycle protein P 16 and retinoblastoma and c-fos in the cerebral cortex of senile mice].
Zhen Ci Yan Jiu
; 35(4): 250-4, 276, 2010 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-21090325
ABSTRACT
OBJECTIVE:
To observe the influence of moxibustion on the cyclin and cellular proliferin of the cerebral cortex in senile mice so as to explore its underlying mechanism in delaying aging.METHODS:
Sixty male mice were randomly and equally divided into control, model, moxibustion of "Zusanli" (ST 36) and "Xuanzhong" (GB 39, M-ST 36-GB 39), moxibustion of "Baihui" (GV 20) and "Guanyuan" (CV 4,M-GV 20-COV 4) ,and medication groups. The aging model was established by subcutaneous injection of D-galactose for 42 days. Moxibustion was applied to ST 36, GB 39, GV 20 and CV 4 separately for 3 moxa-cones, once every other day for one month. The expression of cell cycle protein P 16 and retinoblastoma (pRb), and c-fos protein in the cerebral cortex tissue of the senile mice were detected by immunohistochemistry.RESULTS:
Compared with control group, the number of P16 immunoreaction (IR) positive neurons in the cerebral cortex increased significantly in the model group (P < 0.01), and those of cortical pRb and c-fos IR-positive neurons decreased considerably in model group (P < 0.01). In comparison with the model group, the number of cortical P 16 IR-positive neurons reduced significantly in M-ST 36-GB 39, M-GV 20-CV 4 and medication groups (P < 0.01), and those of cortical pRb and c-fos IR-positive neurons increased remarkably in M-ST 36-GB 39, M-GV 20-CV 4 and medication groups (P < 0.01, P < 0.05). No significant differences were found in the aforementioned 3 indexes among M-ST 36-GB 39, M-GV 20-CV 4 and medication groups (P > 0.05).CONCLUSION:
Moxibustion of ST 36-GB 39 and GV 20-CV 4 can down-regulate the P 16 expression,and up-regulate pRb and c-fos protein expression in the cerebral cortex of senile mice, which possibly contributes to its effect in delaying aging.
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Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_energeticas
Assunto principal:
Envelhecimento
/
Expressão Gênica
/
Córtex Cerebral
/
Proteína do Retinoblastoma
/
Proteínas Proto-Oncogênicas c-fos
/
Inibidor p16 de Quinase Dependente de Ciclina
/
Moxibustão
Tipo de estudo:
Clinical_trials
Idioma:
Zh
Revista:
Zhen Ci Yan Jiu
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China