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[Effects of moxibustion on the expression of cell cycle protein P 16 and retinoblastoma and c-fos in the cerebral cortex of senile mice].
Du, Yan; Zhao, Li-Hua; Wu, Hai-Biao; Wang, Jin-Sheng.
Afiliação
  • Du Y; Department of Acu-moxibustion, the Affiliated Ruikang Hospital of Guangxi College of Chinese Medicine, Nanning 530011, China. gxduyan@163.com
Zhen Ci Yan Jiu ; 35(4): 250-4, 276, 2010 Aug.
Article em Zh | MEDLINE | ID: mdl-21090325
ABSTRACT

OBJECTIVE:

To observe the influence of moxibustion on the cyclin and cellular proliferin of the cerebral cortex in senile mice so as to explore its underlying mechanism in delaying aging.

METHODS:

Sixty male mice were randomly and equally divided into control, model, moxibustion of "Zusanli" (ST 36) and "Xuanzhong" (GB 39, M-ST 36-GB 39), moxibustion of "Baihui" (GV 20) and "Guanyuan" (CV 4,M-GV 20-COV 4) ,and medication groups. The aging model was established by subcutaneous injection of D-galactose for 42 days. Moxibustion was applied to ST 36, GB 39, GV 20 and CV 4 separately for 3 moxa-cones, once every other day for one month. The expression of cell cycle protein P 16 and retinoblastoma (pRb), and c-fos protein in the cerebral cortex tissue of the senile mice were detected by immunohistochemistry.

RESULTS:

Compared with control group, the number of P16 immunoreaction (IR) positive neurons in the cerebral cortex increased significantly in the model group (P < 0.01), and those of cortical pRb and c-fos IR-positive neurons decreased considerably in model group (P < 0.01). In comparison with the model group, the number of cortical P 16 IR-positive neurons reduced significantly in M-ST 36-GB 39, M-GV 20-CV 4 and medication groups (P < 0.01), and those of cortical pRb and c-fos IR-positive neurons increased remarkably in M-ST 36-GB 39, M-GV 20-CV 4 and medication groups (P < 0.01, P < 0.05). No significant differences were found in the aforementioned 3 indexes among M-ST 36-GB 39, M-GV 20-CV 4 and medication groups (P > 0.05).

CONCLUSION:

Moxibustion of ST 36-GB 39 and GV 20-CV 4 can down-regulate the P 16 expression,and up-regulate pRb and c-fos protein expression in the cerebral cortex of senile mice, which possibly contributes to its effect in delaying aging.
Assuntos
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Base de dados: MEDLINE Métodos Terapêuticos e Terapias MTCI: Terapias_energeticas Assunto principal: Envelhecimento / Expressão Gênica / Córtex Cerebral / Proteína do Retinoblastoma / Proteínas Proto-Oncogênicas c-fos / Inibidor p16 de Quinase Dependente de Ciclina / Moxibustão Tipo de estudo: Clinical_trials Idioma: Zh Revista: Zhen Ci Yan Jiu Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China
Buscar no Google
Base de dados: MEDLINE Métodos Terapêuticos e Terapias MTCI: Terapias_energeticas Assunto principal: Envelhecimento / Expressão Gênica / Córtex Cerebral / Proteína do Retinoblastoma / Proteínas Proto-Oncogênicas c-fos / Inibidor p16 de Quinase Dependente de Ciclina / Moxibustão Tipo de estudo: Clinical_trials Idioma: Zh Revista: Zhen Ci Yan Jiu Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China