The ODN probes conjugating the Cu(II) complex enhance the luminol chemiluminescence by assembling on the DNA template.
Bioorg Med Chem
; 18(24): 8614-7, 2010 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-21115284
ABSTRACT
Potent peroxidase-like activity of the ß-ketoenamine (1)-dicopper (II) complex (2) for the chemiluminescence (CL) of luminol either in the presence or absence of H(2)O(2) has been previously demonstrated by our group. In this study, the ß-ketoenamine (1) as the ligand unit for copper(II) was incorporated into the oligonucleotide (ODN) probes. It has been shown that the catalytic activity of the ODN probes conjugating the ligand-Cu(II) complex is activated by hybridization with the target DNA with the complementary sequence. Thus, this study has successfully demonstrated the basic concept for the sensitive detection of nucleic acids by CL based on the template-inductive activation of the catalytic unit for CL.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Moldes Genéticos
/
DNA
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Sondas de Oligonucleotídeos
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Cobre
/
Medições Luminescentes
Idioma:
En
Revista:
Bioorg Med Chem
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão