[Molecular identification of geminivirus inducing vein yellowing in Abelmoschus manihot].

ABSTRACT

OBJECTIVE: The virus isolate H was identified by molecular biology,it was collected from Abelmoschus manihot plant showing leaf curl,yellow vein symptoms in Guangxi Botanical Garden of Medicinal Plant. METHODS: The virus isolate H was observed in electron micrograph, and conformed detected by PCR using universal primer pair for the genus Geminivirus. RESULTS: The results indicated that all sequences homologous to the specific fragment belonged to the genus Begomovirus of the family Geminiviridae. There was the highest similarity shared 95% homology at nucleotide between the specific fragment and DNA-A of Emilia yellow vein virus isolates. CONCLUSION: These findings suggested that there was geminiviridea in Abelmoschus manihot, and the disease probably caused by Emilia yellow vein virus.