Characterization of upregulated genes associated with high phosphorus accumulation in cucumber.
Physiol Plant
; 143(4): 344-54, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21883253
ABSTRACT
Excessive application of phosphorus (P)-rich manures to agricultural lands often results in P-accumulation in soils leading to water pollution through runoffs and leaching. Use of suitable plant species that can extract and sequester excess P from soil into their biomass is an effective method of remediation of P-contaminated soils. Knowledge on the molecular responses of plants to high P-accumulation and tolerance is lacking. Therefore, a suppression subtractive hybridization (SSH) strategy was employed to identify and elucidate the pattern of gene expression related to P-tolerance and accumulation in cucumber (Cucumis sativus L.), a P-accumulator plant. RNA isolated from cucumber grown in high P was used for 'tester' cDNA synthesis and SSH library preparation. A total of 63 cDNAs were identified as showing upregulated expression in this plant in response to high P. No putative function could be assigned to 7 (11%) of the 63 upregulated high P-modulated genes and 11 expressed sequence tags (ESTs) (17%) did not match database entries. The remaining 45 ESTs were grouped into five functional classes. The majority of these ESTs belonged to three groups 'metabolism', 'protein synthesis/degradation and signaling' and 'cell structure/cell wall'. Only six 'stress/defense'-related ESTs were identified from this library. The results of reverse northern blot analysis was further confirmed and validated through semi-quantitative RT-PCR carried out with representative ESTs identified in this study. The research reported here may contribute to a preliminary understanding of the high P-related gene expression in this P-accumulating plant.
Texto completo:
1
Base de dados:
MEDLINE
Medicinas Complementares:
Homeopatia
Assunto principal:
Fósforo
/
Genes de Plantas
/
Cucumis sativus
/
Etiquetas de Sequências Expressas
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Physiol Plant
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Estados Unidos