[Bioaugmentation of bioreactors with a pJP4 receiving transconjugant to enhance the removal of 2,4-D].
Huan Jing Ke Xue
; 32(7): 2152-7, 2011 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-21922845
ABSTRACT
The paper first investigated horizontal transfer of a conjugative plasmid pJP4 to two pure strains of E. coli DH5alpha and Alcaligenes sp., and a mixed culture of aerobic granular sludge, respectively. With a pJP4 receiving transconjugant Alcaligenes sp. pJP4 as the bioaugmented bacteria, bioaugmentation experiments were conducted in an aerobic granular sludge reactor and a biofilm reactor, respectively, to enhance the removal of a recalcitrant compound 2,4-dichlorophenoxyacetic acid(2,4-D). Results showed that pJP4 successfully transferred to E. coli DH5alpha, Alcaligenes sp. and the mixed culture of aerobic granules. For the aerobic granular sludge reactor operated in semi-continuous mode and fed with 2,4-D sole carbon source wastewater, bioaugmentation with Alcaligenes sp. pJP4 increased 2,4-D average removal rate significantly with an enhancement of 12% -1 498%. For the biofilm reactor operated in sequence batch mode and fed with mixed carbon sources wastewater, supplementation of the transconjugant reduced system start-up time greatly from 16 d to 5 d. It is a feasible strategy to obtain special degradative transconjugants through gene augmentation and put them into bioreactor as bioaugmentation agent to enhance the removal of some specific pollutants.
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Base de dados:
MEDLINE
Assunto principal:
Eliminação de Resíduos Líquidos
/
Reatores Biológicos
/
Ácido 2,4-Diclorofenoxiacético
Idioma:
Zh
Revista:
Huan Jing Ke Xue
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China