A novel sesquiterpene acid and an alkaloid from leaves of the Eastern Nigeria mistletoe, Loranthus micranthus with potent immunostimulatory activity on C57BL6 mice splenocytes and CD69 molecule.
Pharm Biol
; 49(12): 1271-6, 2011 Dec.
Article
em En
| MEDLINE
| ID: mdl-21988279
ABSTRACT
CONTEXT The Eastern Nigeria mistletoe, Loranthus micranthus Linn. (Loranthaceae), is used in the treatment of several diseases including immune-modifying diseases and thus there is a need to identify the immunoactive constituents. OBJECTIVE:
This research isolated and characterized the immunoactive constituents in the Eastern Nigeria mistletoe. MATERIALS ANDMETHODS:
Bioassay-guided fractionation was employed in the isolation and purification of the constituents. The characterized compounds were screened for immunostimulatory activities on isolated C57BL/6 mice splenocytes and early activation marker, CD69 at concentrations of 10, 25, and 100 µg/mL using flow cytometry techniques and compared to lipopolysaccharide (LPS; 10 µg/mL) and concanavalin A (ConA; 2 µg/mL) as standards.RESULTS:
Two compounds, a novel sesquiterpene, 2, 3-dimethoxy-benzo [a, b] cyclopentenyl-3',3',5'-trimethyl pyran-4-carboxylic acid, and a known alkaloid, lupinine were isolated and characterized. The compounds (25 µg/mL) showed statistically significantly (p < 0.05) stimulatory activity on the splenocytes with values of 56.34 ± 0.26% and 69.84 ± 0.19%, respectively, compared to 7.58 ± 0.42% recorded for the unstimulated control. Similarly, the CD69 expression assay showed immunostimulation with statistically significant values (p < 0.05) of 2.31 ± 0.07% and 2.71 ± 0.03%, respectively, compared to 1.69 ± 0.05% recorded for the nonstimulated control.DISCUSSION:
These data suggest that the isolated compounds possess immunomodifying abilities. In addition, the activation of the CD69 molecule is possibly one of its mechanisms of action.CONCLUSION:
These compounds may be responsible in part, for the immunostimulatory activities already established for the Eastern Nigeria mistletoes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sesquiterpenos
/
Baço
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Antígenos de Diferenciação de Linfócitos T
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Antígenos CD
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Adjuvantes Imunológicos
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Loranthaceae
/
Lectinas Tipo C
/
Alcaloides
Tipo de estudo:
Guideline
/
Qualitative_research
País/Região como assunto:
Africa
Idioma:
En
Revista:
Pharm Biol
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Nigéria