Development of an enzyme-linked immunosorbent assay (ELISA) for residue analysis of the fungicide azoxystrobin in agricultural products.
J Agric Food Chem
; 60(4): 904-11, 2012 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-22224459
ABSTRACT
A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for residue analysis of azoxystrobin in garden crops, for which the maximum residue limits (MRLs) are 0.5-50 mg/kg in Japan. For hapten synthesis, an ethyl carboxyl group was introduced to the 4-position of the 2-cyanophenoxy group in azoxystrobin, and its cyano group was changed to a methyl group. An anti-azoxystrobin monoclonal antibody was prepared from mice immunized with hapten-keyhole limpet hemocyanin conjugate. The dc-ELISA using prepared antibody showed 50-250-fold higher sensitivity compared to the MRLs. The working range of the dc-ELISA was 10-200 ng/mL. The dc-ELISA showed high specificity to azoxystrobin. When methanol extracts from nine kinds of garden crops spiked with azoxystrobin ranging near the MRLs were analyzed, the determined results by the dc-ELISA agreed well with the results of their controls. In addition, azoxystrobin spiked in garden crops homogenates was satisfactorily extracted by methanol solution and easily analyzed. The recovery rate of dc-ELISA was 96-109% and correlated well with the results obtained by HPLC analysis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pirimidinas
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Ensaio de Imunoadsorção Enzimática
/
Resíduos de Praguicidas
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Produtos Agrícolas
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Fungicidas Industriais
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Metacrilatos
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Japão