Assessment of in vitro antioxidant, antibacterial and immune activation potentials of aqueous and ethanol extracts of Phyllanthus niruri.

BACKGROUND: Recently much attention has been paid to biologically active plants because of their low production cost and fewer adverse effects compared with chemical drugs. In the present investigation the bioactivity of Phyllanthus niruri ethanol and aqueous extracts was evaluated in vitro. RESULTS: The ethanol extract of P. niruri showed a high level of flavonoid content (123.9 ± 0.002 mg g⁻¹), while the aqueous extract showed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH; IC50 6.85 ± 1.80 µmol L⁻¹) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS; 46.44 ± 0.53 µmol L⁻¹) free radical scavenging activities with high phenol content (376 ± 0.02 mg g⁻¹) and elevated levels of ferric reducing antioxidant power (FRAP; 23 883 ± 0.019 mmol g⁻¹) with excellent antibacterial activity against Staphylococcus aureus (20 mm inhibition zone) and Streptococcus agalactiae (12 mm inhibition zone), respectively, in addition to the best immune activation potential of human peripheral blood mononuclear cells (450.5%). CONCLUSIONS: It is clear from our results that both extracts of P. niruri has excellent bioactivity roles via elevated levels of antibacterial, antioxidant and percentage of peripheral blood mononuclear cell proliferation, which could lead to the development of medications for clinical use.