In-vitro evaluation of anti-trichomonal activities of Eugenia uniflora leaf.
Afr J Tradit Complement Altern Med
; 8(2): 170-6, 2011.
Article
em En
| MEDLINE
| ID: mdl-22238499
ABSTRACT
Eugenia uniflora, used ethnomedically in some tropical countries as an anti-infective, has shown anti-malarial and anti-trypanocidal activities. Therefore using bioactivity guided fractionation, anti-trichomonal activity of E. uniflora leaf was investigated. Anti-trichomonal activities of leaf methanol extract and its fractions against Trichomonas gallinae as well as their cytotoxicities using an in vitro haemaglutination assay were determined. Anti-trichomonacidal activities of the extract improved on purification up to a stage. Subfractions E(2-5) had LC(50) and LC(90) values of 4.77 - 5.28, 18.49 - 25.00 and 4.53 - 5.18, 18.32 - 19.07 µg/ml at 24 and 48 hrs, respectively that were better than those of metronidazole. Further purification of E(2-5) led to loss of activity suggesting that the active components were probably working synergistically and additively. Demonstration of low haemaglutination titre values of 0.00 - 5.33 by methanolic extract and its partition fractions suggested their low toxicity profile. The established safety of the leaf indicated that its anti-trichomonal activity was not due to non-specific cytotoxicity, hence could be used in ethnomedicine as an anti-trichomonal agent.
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Texto completo:
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Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
/
Aromoterapia
/
Plantas_medicinales
Assunto principal:
Trichomonas
/
Extratos Vegetais
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Folhas de Planta
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Syzygium
/
Fitoterapia
/
Antitricômonas
Idioma:
En
Revista:
Afr J Tradit Complement Altern Med
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Nigéria