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Anti-inflammatory and anti-allergic effects of Agrimonia pilosa Ledeb extract on murine cell lines and OVA-induced airway inflammation.
Kim, Jae-Jin; Jiang, Jun; Shim, Do-Wan; Kwon, Sang-Chul; Kim, Tack-Joong; Ye, Sang-Kyu; Kim, Myong-Ki; Shin, Yong-Kook; Koppula, Sushruta; Kang, Tae-Bong; Choi, Dong-Kug; Lee, Kwang-Ho.
Afiliação
  • Kim JJ; Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Chungju 380-701, Republic of Korea.
J Ethnopharmacol ; 140(2): 213-21, 2012 Mar 27.
Article em En | MEDLINE | ID: mdl-22289347
ABSTRACT
ETHNOPHARMACOLOGICAL EVIDENCE Agrimonia pilosa Ledeb (Rosaceae, AP) has long been used as a traditional medicine in Korea and other Asian countries to treat various diseases. AIM OF THE STUDY In the present study, the anti-inflammatory and anti-allergic effects of AP extract in in vitro cell lines and in vivo mouse model of inflammation and the molecular mechanisms involved were reported. MATERIALS AND

METHODS:

Using Raw 264.7 murine macrophages the effects of methanol extract of AP in lipopolysaccharide (LPS)-induced production of inflammatory mediators were measured. Further IgE-DNP-induced interleukin (IL)-4 production and degranulation in RBL-2H3 rat basophilic cell lines was also estimated. To investigate the anti-asthmatic effect of AP in vivo, airway inflammation in ovalbumin (OVA)-induced mouse model was used.

RESULTS:

AP attenuated the production of inflammatory mediators such as NO, PGE(2) and pro-inflammatory cytokines in LPS-induced Raw 264.7 cells. Further, AP inhibited IL-4 production and degranulation in IgE-DNP-induced RBL-2H3 cells. Furthermore, AP attenuated the infiltration of immune cells into lung, cytokines production in broncho-alveolar lavage fluid (BALF) and airway-hyperresponsiveness (AHR) on OVA-induced mouse model of inflammation.

CONCLUSION:

Our results showed that AP attenuated the activation of macrophages, basophils, and inhibited the OVA-induced airway inflammation. The molecular mechanisms leading to AP's potent anti-inflammatory and anti-allergic effects might be through regulation of TRIF-dependent and Syk-PLCγ/AKT signaling pathways, suggesting that AP may provide a valuable therapeutic strategy in treating various inflammatory diseases including asthma.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Métodos Terapêuticos e Terapias MTCI: Terapias_biologicas Assunto principal: Asma / Citocinas / Mediadores da Inflamação / Antialérgicos / Agrimonia / Fitoterapia / Anti-Inflamatórios Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2012 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Métodos Terapêuticos e Terapias MTCI: Terapias_biologicas Assunto principal: Asma / Citocinas / Mediadores da Inflamação / Antialérgicos / Agrimonia / Fitoterapia / Anti-Inflamatórios Idioma: En Revista: J Ethnopharmacol Ano de publicação: 2012 Tipo de documento: Article