Effects of supplemental dietary arginine on the exogenous advanced glycosylation end product-induced interleukin-23/interleukin-17 immune response in rats.
Nutrition
; 28(10): 1063-7, 2012 Oct.
Article
em En
| MEDLINE
| ID: mdl-22673595
ABSTRACT
OBJECTIVES:
Arginine (Arg) is known to possess numerous useful physiological properties and immunomodulatory effects. Th17 cells are a unique T-helper cell lineage. Regulation of Th17 cells plays a significant role in the pathogenesis of inflammatory disorders. This study investigated the effect of Arg on the exogenous advanced glycosylation end product (AGE)-induced Th17-mediated immune response.METHODS:
Rats were randomly divided into three groups. The control BSA (CB) group was fed a common diet and given a tail vein injection of non-glycated bovine serum albumin (BSA). The control AGE (CA) group was fed the common diet and injected with 2 mg AGE-BSA. Arg-AGE (AA) group was fed the Arg-supplemented diet and injected with 2 mg AGE-BSA. The experimental diets were identical in energy and nutrient distributions except for the amino acid content. Arg provided 2% of the total energy. Tail vein injections and diets were given daily. After 10 d, all rats were sacrificed, and blood samples were collected for further analysis.RESULTS:
The AA group had the highest inducible nitric oxide (NO) synthase expression and plasma NO levels. The percentage of Foxp3 T-regulatory cells in the AA group was lower than those of the CA and CB groups. Transforming growth factor-ß1, interleukin (IL)-6, and IL-17A gene expression was higher in the AGE-administered groups. The AA group had higher TGF-ß1 and IL-17A expression than did the CA group.CONCLUSION:
These results suggest that in a condition of exogenous AGE administration, supplemental dietary Arg resulted in a more pronounced IL-23/IL-17 immune response, possibly by increasing NO secretion.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Arginina
/
Produtos Finais de Glicação Avançada
/
Interleucina-17
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Interleucina-23
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Células Th17
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Fatores Imunológicos
/
Inflamação
Idioma:
En
Revista:
Nutrition
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Taiwan