Application of HPLC-NMR in the identification of plocamenone and isoplocamenone from the marine red alga Plocamium angustum.
Mar Drugs
; 10(9): 2089-2102, 2012 Sep.
Article
em En
| MEDLINE
| ID: mdl-23118723
ABSTRACT
A combination of on-line HPLC-NMR and off-line chemical investigations has resulted in the identification of the previously reported polyhalogenated monoterpene plocamenone, together with the new structural analogue isoplocamenone from the crude extract of the marine alga Plocamium angustum. On-flow and stop-flow HPLC-NMR analyses (including the acquisition of WET 2D NMR spectra) rapidly assisted in the identification of the major component plocamenone and in the partial identification of its unstable double bond isomer isoplocamenone. Conventional off-line isolation and structural characterization techniques were employed to unequivocally confirm both structures, leading to a structural revision for plocamenone, as well as to obtain sufficient quantities for biological testing.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Espectroscopia de Ressonância Magnética
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Cromatografia Líquida de Alta Pressão
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Monoterpenos
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Plocamium
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Mar Drugs
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Austrália