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Toxoflavin lyase enzyme as a marker for selecting potato plant transformants.
Kim, Mi-Sun; Kim, HyunSoon; Moon, Jae Sun; Hwang, Ingyu; Joung, Hyouk; Jeon, Jae-Heung.
Afiliação
  • Kim MS; Green-Bio Research Center, KRIBB, Daejeon, Korea.
Biosci Biotechnol Biochem ; 76(12): 2354-6, 2012.
Article em En | MEDLINE | ID: mdl-23221711
This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.
Assuntos
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Base de dados: MEDLINE Assunto principal: Pirimidinonas / Transformação Genética / Triazinas / Solanum tuberosum / Engenharia Genética / Marcadores Genéticos / Liases Idioma: En Revista: Biosci Biotechnol Biochem Ano de publicação: 2012 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Pirimidinonas / Transformação Genética / Triazinas / Solanum tuberosum / Engenharia Genética / Marcadores Genéticos / Liases Idioma: En Revista: Biosci Biotechnol Biochem Ano de publicação: 2012 Tipo de documento: Article