Effect of ozone therapy on autogenous bone graft healing in calvarial defects: a histologic and histometric study in rats.
J Periodontal Res
; 48(6): 722-6, 2013 Dec.
Article
em En
| MEDLINE
| ID: mdl-23452156
ABSTRACT
AIM:
The purpose of this study was to analyze histologically the effect of ozone therapy in combination with autogenous bone graft on bone healing in rat calvaria.METHODS:
Critical size defects were created in calvaria of 27 male Wistar rats. The animals were divided into three groups of nine animals each autogenous bone graft group (n = 9); autogenous bone graft with ozone therapy group (80%, 30 s 3 d for 2 wk, n = 9); non-treatment (control) group (n = 9). Animals were killed after 8 wk. Histomorphometric assessments, using image analysis software, and histological analyses were performed. Primary outcome was total bone area. Secondary outcomes (osteoblast number, new bone formation) were also measured.RESULTS:
Histomorphometrically, the total bone area in the autogenous bone graft with ozone therapy group (9.3 ± 2.2) were significantly higher than that of the autogenous bone graft group (5.1 ± 1.8) (p < 0.05). Also, the ozone therapy group significantly increased the percentage of total bone area compared to the autogenous bone graft group (p < 0.05). The osteoblast number significantly increased in the autogenous bone graft with the ozone therapy group (58 ± 12.3) compared to the autogenous bone graft group (9.3 ± 3.5) (p < 0.05). Also, it was observed that autogenous bone graft with ozone therapy group showed significant new bone formation when compared to the autogenous bone graft group (p < 0.05).CONCLUSION:
Ozone therapy enhances new bone formation by autogenous bone graft in the rat calvarial defect model.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
/
Ozonoterapia
Assunto principal:
Ozônio
/
Crânio
/
Doenças Ósseas
/
Transplante Ósseo
/
Autoenxertos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Periodontal Res
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Turquia