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[Molecular identification of astragali radix and its adulterants by ITS sequences].
Cui, Zhan-Hu; Li, Yue; Yuan, Qing-Jun; Zhou, Li-She; Li, Min-Hui.
Afiliação
  • Cui ZH; Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi ; 37(24): 3773-6, 2012 Dec.
Article em Zh | MEDLINE | ID: mdl-23627177
OBJECTIVE: To explore a new method for identification Astragali Radix from its adulterants by using ITS sequence. METHOD: Thirteen samples of the different Astragali Radix materials and 6 samples of the adulterants of the roots of Hedysarum polybotrys, Medicago sativa and Althaea rosea were collected. ITS sequence was amplified by PCR and sequenced unidirectionally. The interspecific K-2-P distances of Astragali Radix and its adulterants were calculated, and NJ tree and UPGMA tree were constructed by MEGA 4. RESULT: ITS sequences were obtained from 19 samples respectively, there were Astragali Radix 646-650 bp, H. polybotrys 664 bp, Medicago sativa 659 bp, Althaea rosea 728 bp, which were registered in the GenBank. Phylogeny trees reconstruction using NJ and UPGMA analysis based on ITS nucleotide sequences can effectively distinguish Astragali Radix from adulterants. CONCLUSION: ITS sequence can be used to identify Astragali Radix from its adulterants successfully and is an efficient molecular marker for authentication of Astragali Radix and its adulterants.
Assuntos
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Base de dados: MEDLINE Assunto principal: DNA Ribossômico / Raízes de Plantas / DNA de Plantas / Astragalus propinquus Tipo de estudo: Diagnostic_studies Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Ano de publicação: 2012 Tipo de documento: Article País de afiliação: China
Buscar no Google
Base de dados: MEDLINE Assunto principal: DNA Ribossômico / Raízes de Plantas / DNA de Plantas / Astragalus propinquus Tipo de estudo: Diagnostic_studies Idioma: Zh Revista: Zhongguo Zhong Yao Za Zhi Ano de publicação: 2012 Tipo de documento: Article País de afiliação: China