[Molecular identification and detection of moon jellyfish (Aurelia sp.) based on partial sequencing of mitochondrial 16S rDNA and COI].
Ying Yong Sheng Tai Xue Bao
; 24(3): 847-52, 2013 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-23755504
ABSTRACT
Taking the moon jellyfish Aurelia sp. commonly found in our coastal sea areas as test object, its genome DNA was extracted, the partial sequences of mt-16S rDNA (650 bp) and mt-COI (709 bp) were PCR-amplified, and, after purification, cloning, and sequencing, the sequences obtained were BLASTn-analyzed. The sequences of greater difference with those of the other jellyfish were chosen, and eight specific primers for the mt-16S rDNA and mt-COI of Aurelia sp. were designed, respectively. The specificity test indicated that the primer AS3 for the mt-16S rDNA and the primer AC3 for the mt-COI were excellent in rapidly detecting the target jellyfish from Rhopilema esculentum, Nemopilema nomurai, Cyanea nozakii, Acromitus sp., and Aurelia sp., and thus, the techniques for the molecular identification and detection of moon jellyfish were preliminarily established, which could get rid of the limitations in classical morphological identification of Aurelia sp. , being able to find the Aurelia sp. in the samples more quickly and accurately.
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Base de dados:
MEDLINE
Assunto principal:
DNA Ribossômico
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Proteínas Mitocondriais
/
Cifozoários
/
Mitocôndrias
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Ying Yong Sheng Tai Xue Bao
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China