[Isolation and mass spectrometric analysis of antioxidant peptides from enzymatic hydrolysates of roasted pills of Asini Corii Colla].
Zhongguo Zhong Yao Za Zhi
; 38(7): 1021-5, 2013 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-23847950
ABSTRACT
A fraction named GFC-1 with high antioxidant activities in vitro was isolated from the enzymatic hydrolysates of roasted pills of Asini Corii Colla, and the peptides in this fraction were identified. The enzymatic hydrolysates were isolated and purified with anion exchange chromatography and Sephadex G-25 filtration chromatography successively. GFC-1, a fraction isolated from the hydrolysates, exhibited the highest DPPH and ABTS scavenging capacity (DPPH 47. 95% at 2.0 g x L(-1) and ABTS 97.20% at 0.40 g x L(-1). Nine peptides from GFC-1 were identified by LC-ESI-MS/MS coupled with TurboSEQUEST search software and Swiss-Prot data base, and a high repetition core sequence GPAGPP*GPP* was also found.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Hidrolisados de Proteína
/
Pele
/
Antioxidantes
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China