Effect of curcumin on down-expression of thrombospondin-4 induced by oxidized low-density lipoprotein in mouse macrophages.
Biomed Mater Eng
; 24(1): 181-9, 2014.
Article
em En
| MEDLINE
| ID: mdl-24211897
ABSTRACT
This study was designed to investigate the effect of curcumin on the expression of thrombospondin-4 (THBS-4) in mouse macrophages treated with oxidized low-density lipoprotein (oxLDL). The mouse macrophage cell line ANA-1 was treated with oxLDL. Cell viability was measured by MTT assay. ANA-1 cells were divided into five groups control group, model group, 5 µM curcumin group, 15 µM curcumin group and 25 µM curcumin group. The gene and protein expression levels of THBS-4 in each group were determined by real-time quantitative polymerase chain reaction (PCR) and western blotting, respectively. MTT assay showed that curcumin concentrations up to 25 µM and oxLDL concentrations up to 20 µg/ml had no significant cytotoxic effects on macrophages at 24 h. Real-time quantitative PCR revealed that THBS-4 mRNA expression was markedly reduced by stimulation with oxLDL, but subsequently significantly increased by treatment with curcumin. Western blotting confirmed that curcumin (5, 15, and 25 µM) significantly prevented the decrease in THBS-4 expression induced by oxLDL (20 µg/ml) in macrophages. Curcumin prevents the decrease in THBS-4 expression induced by oxLDL, which may represent one of the anti-atherosclerotic mechanisms of curcumin.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
/
Aromoterapia
/
Plantas_medicinales
Assunto principal:
Regulação para Baixo
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Trombospondinas
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Curcumina
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Lipoproteínas LDL
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Macrófagos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biomed Mater Eng
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
China