Influence of extract of Ginkgo biloba leaves tablets on the aquaporin-1 expression in isolated lung ischemia reperfusion.
Chin Med J (Engl)
; 126(24): 4720-3, 2013.
Article
em En
| MEDLINE
| ID: mdl-24342318
ABSTRACT
BACKGROUND:
The extract of Ginkgo biloba leaves tablets, ginaton, is widely used in treating ischemic cerebrovascular disease in the clinic. This study aimed to investigate the expression of aquaporin-1 (AQP-1) in rat lung with ischemia/reperfusion injury after pretreatment with ginaton, and whether the pretreatment with ginaton reduces the acute lung injury caused by ischemia/reperfusion injury.METHODS:
Adult Wistar rats were divided into two groups. Some rats were used as donors (n = 20), the others as recipients (n = 20). Left lungs of donor rats were used for the isolated lung reperfusion model, which perfused only with low potassium dextran (LPD) solution as group A (n = 10); the others were pretreated with ginaton before reperfusion as group C (n = 10). Right lung of donor rat without any treatment was used as a control group (group B and group D, n = 10 for each group). After the model was established, the expression of AQP-1 in the lung tissues was examined by immunohistochemistry, Western blotting, and reverse transcriptase-polymerase chain reaction.RESULTS:
Immunohistochemical examination revealed that AQP-1 was expressed in endothelia. Immunoblotting demonstrated that the relative gray values of AQP-1 protein in groups A and C were 0.65±0.06, 0.88±0.11, respectively. The relative gray values of the mRNA expression in groups A and C were 0.30±0.08, 0.49±0.11, respectively. The expression of AQP-1 protein and mRNA in group C was significantly higher than in group A (P < 0. 05).CONCLUSION:
The pretreatment with ginaton can reduce the acute lung injury caused by ischemia/reperfusion.
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Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
/
Folhas de Planta
/
Ginkgo biloba
/
Aquaporina 1
/
Pulmão
Idioma:
En
Revista:
Chin Med J (Engl)
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
China