LBP-4a improves insulin resistance via translocation and activation of GLUT4 in OLETF rats.
Food Funct
; 5(4): 811-20, 2014 Apr.
Article
em En
| MEDLINE
| ID: mdl-24577527
ABSTRACT
Lycium barbarum polysaccharide (LBP) has been shown to ameliorate insulin resistance, but the identification of compounds from LBP and the mechanisms have not been clarified. In this study, LBP-4a was purified from Lycium barbarum by DEAE cellulose and Sephadex G-100 column chromatography, and the effects of LBP-4a on insulin resistance were investigated. The results indicated that LBP-4a caused translocation of the glucose transporter isoform 4 (GLUT4) to the cell surface, which in turn stimulated glucose uptake, and the effect was sensitive to wortmannin, an inhibitor of phosphoinositol 3-kinase (PI3-K), and SB203580, an inhibitor of p38 mitogen activated protein kinase (p38 MAPK (α, ß)). Furthermore, the effects of LBP-4a on p38 MAPK activities were abrogated by pretreatment of rat adipocytes using SB203580. In summary, LBP-4a improved insulin resistance via translocation and activation of GLUT4 in OLETF rats, and the activation of PI3-K and p38 MAPK contributed to these effects.
Texto completo:
1
Base de dados:
MEDLINE
Medicinas Tradicionais:
Medicinas_tradicionales_de_asia
/
Medicina_china
Assunto principal:
Resistência à Insulina
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Medicamentos de Ervas Chinesas
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Lycium
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Diabetes Mellitus Tipo 2
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Transportador de Glucose Tipo 4
Idioma:
En
Revista:
Food Funct
Ano de publicação:
2014
Tipo de documento:
Article