[Identification of radix et rhizoma clematidis and its adulterants using DNA barcoding].
Yao Xue Xue Bao
; 49(2): 260-6, 2014 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-24761620
ABSTRACT
This study provides the candidate sequences in the identification of Radix et Rhizoma Clematidis and its adulterants using DNA barcoding. We amplified and sequenced the region psbA-trnH, with the data of 284 sequences from GenBank, the differential intra- and inter-specific divergences, genetic distance, barcoding gap were used to evaluate five barcodes, and the identification efficiency was assessed using BLAST1 and Nearest Distance methods. The results showed that psbA-trnH barcodes performed high identification efficiency and inter-specific divergences among the five different DNA barcodes. Analysis of the barcoding gap and NJ tree showed psbA-trnH was superior to other barcodes. Based on the identification and PCR amplification efficiency, psbA-trnH can be the ideal barcode to identify Radix et Rhizoma Clematidis and its adulterants accurately.
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Base de dados:
MEDLINE
Métodos Terapêuticos e Terapias MTCI:
Terapias_biologicas
Assunto principal:
Plantas Medicinais
/
DNA de Plantas
/
Ranunculaceae
/
Código de Barras de DNA Taxonômico
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Ano de publicação:
2014
Tipo de documento:
Article